BANGLADESH JOURNAL OF BIOCHEMISTRY

Bangladesh Journal of  Biochemistry
AN INTERNATIONAL JOURNAL IN BIOCHEMICAL SCIENCE

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Bangladesh Journal of Biochemistry. Vol. 4, No. 1&2, 1-11, 1998
ISOLATION AND CHARACTERIZATION OF A THIRD RIBOSOME INACTIVATING PROTEIN, LUFFIN-C, FROM SPONGE GOURD (LUFFA CYLINDRICA) SEEDS AN IMPROVED METHOD FOR PURIFICATION OF LUFFIN VARIANTS 

M. Rafiqul ISLAM,
Present address: Department of Chemistry, Northwest Missouri State University, 800 University Drive, Maryville, MO 64468, USA.
Keiichi WATANABE,
Present address: Department of Applied Biological Science, Saga University, Saga 840, Japan.
(Laboratory of  Protein Chemistry and Engineering, Faculty of Agriculture, Kyushu University, Fukuoka 812, Japan.)

Received January 15, 1998 

Abstract
Previously two ribosome-inactivating proteins, named luffin-a and b,  were isolated from the seeds of luffa cylindrica (Kamensono et al, 1988). A new ribosome-inactivating protein, which we named luffin-c has been purified to homogeneity from a salt-acid extract of sponge gourd seeds by chromatography on an AF-Blue Toyopearl column followed by a fast protein liquid chromatography (FPLC) on a Mono-S column. Luffin c, which has a similar molecular mass (28 kDa) and isoelectric point (>10) as those of luffin-a and b, showed a distinct elution time in FPLC Mono-S column. In addition to isolate luffin-c, this rapid purification method improved  the isolation of both luffin-a and b. Amino acid and carbohydrate composition analyses showed that is similar to luffin-a in glycine, alanine, isoleucine and carbohydrate contents, while it is similar to luffin-b in threonine, proline and methionine contents, but its serine content is unusually low than the serine contents of both luffin-a  and b.Comparison of N-terminal sequences of luffin a, -b and c indicated although they are homologous proteins, luffin-c has an extension of two and three residues from the N-terminals of luffin-b and a, respectively. The biological activities of luffin-c, measured by inhibition of protein synthesis in rabbit reticulocyte lysates and by inactivation of rat liver ribosomes, were similar to those of luffin-a. However, both luffin-a and c were found to be 3-4 times stronger in those activities than luffin-b.

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Bangladesh Journal of Biochemistry. Vol. 4, No. 1&2, 13-21, 1998 
VARIATION OF THE BODY COMPOSITION OF HILSHA (TENUALOSA ILISHA) DURING GONAD DEVELOPMENT

Syeda Shamim JAHAN*, Mohammad MUSLEMUDDIN, Syed Fazl-e- RUBBI and Benedict GOMEZ1
Institute of Food Science and Technology, BCSIR,Qudrat-e-Khuda Road., Dhanmondi, Dhaka 1205, Bangladesh.
*Corresponding author.

1
Present address: Department of Biochemistry, Dhaka University, Dhaka.

(Received May 10, 1998) 

Abstract
The proximate composition of hilsha fish (Tenualosa ilisha) varies with the variation of the physiological condition of the fish. The sequences of energy mobilization from net loss of muscle calorie and gain in roe and liver calories were calculated during gonad development. Protein and fat contents in the whole muscle of hilsha decreased with increase in the severity of depletion. The rates of change of these nutrients were different at different  anatomical portions like dorsal, ventral, tail and dark muscle of hilsha. The protein and fat contents of the gonad increased and moisture content decreased with the increase of the size of the roe. The fat content in the liver also increased with no appreciable change in protein content. In a healthy fish maximum amount of energy comes from its muscle (98.4% of total energy) and the roe contributes a very small amount (1.2%). On the other hand, in a depleted fish with matured gonad only 41.7% of the total energy comes from the  muscles, while the roe contributes 56.9%. With the progress of gonad development total energy reserve of the body decreases, due mainly to loss of fat calorie. 

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Bangladesh Journal of Biochemistry. Vol. 4, No. 1&2, 23-31, 1998
CONFORMATION AND SURFACE PROPERTIES OF DEAMIDATED  GLUTEN OF GERMINATING WHEAT

Ranjit Kumar SHAHA 1*, and Akio KATO2
1
Department of Biochemsitry, University of Rajshahi, Rajshahi-6205, Bangladesh and 2Department of Biochemistry, Yamaguchi University, Japan.
*Corresponding author.

(Received May14, 1998)

Abstract
The surface properties of deamidated gluten were investigated with respect to their conformational changes. The helix content of gluten decreased curvilinearly with its decrease of deamidation. The surface tension decresed in proportion to the degree of deamidation. On the other  hand, the surface hydrophobicity of gluten increased remarkably in proportion to the degree of deamidation. The emulsifying properties of gluten were improved greatly by deamidation, correlating linearly with the surface hydrophobicity. From these results, the relationship between  the conformational changes and functional properties of deamidated gluten are discussed.

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Bangladesh Journal of Biochemistry. Vol. 4, No. 1&2, 33-41, 1998
DEVELOPMENT OF LOW-COST MEDIA FOR LARGE SCALE PRODUCTION OF BACILLUS SPHAERICUS

Mahmud HOSSAIN, Nawaz KHAN, M. JAHANGIR, Anwar HOSSAIN, K. A. RAHIM and Mustafizur RAHMAN.

Department of Biochemistry, Dhaka University, Dhaka-1000, Bangladesh

(Received June 16, 1998)

Abstract
Three media formulated from bovine blood serum, soya meal and fish meal in  combination with mineral salts were assessed for the growth of Bacillus sphaericus strains 1593 and SI-I, their sporulation and their toxicity against mosquito larvae. Freeze dried powders of these bacterial strains were found to be active against the larvae of Culex quinquefascitatus (CQF). All the media prepared from indigenous sources performed better than the commercially available chess medium. The highest number of viable cells and spores -8.2108 and 8.1108 respectively for Bacillus sphaericus (1593) and 1.2109and 1.4109 respectively for Bacillus sphaericus SI-1-was found in  bovine serum medium. For CQF, the LC50 value of Bacillus sphaericus SI-1 strain (1g/ml) was reached in 18 hrs. Both Bacillus sphaericus 1593 and Bacillus sphaericus SI-1 strains were tried against the larvae of Aedes also. Only the latter one was found to be active against this larvae, but the activity was extremely poor. Addition of Ca++ in the medium increased the potency of the strains 2-3 times.

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Bangladesh Journal of Biochemistry. Vol. 4, No. 1&2, 43-54, 1998
A COMPARATIVE STUDY ON CHARACTERISTICS OF BOILED AND NON-BOILED TYPES OF RICE BRAN AND THEIR OILS 

M. Mokhlesur RAHMAN, M. Maksud ALI and Nurul ABSAR*
Department of Biochemistry, University of Rajshahi, Rajshahi, Bangladesh

(Received October 26,1998) 

Abstract
Ten samples of boiled and non-boiled types rice bran (BR-14  variety) collected from different rice growing areas of Bangladesh, have been examined for their total lipid (oil), non-lipid components and physico-chemical properties of this total lipid. Minerals content such as calcium, iron and phosphorus were present in good proportion in both boiled and non-boiled types rice bran. Of the major energy producing nutrients, boiled rice bran contained higher amounts of carbohydrates and  fat but non-boiled bran contained higher amount of protein. The total lipid varied from 16.00-18.68%, ash 8.45-9.70%, protein 14.04-15.10% and crude fibre ranged from 4.30-8.92%. Oils extracted from fresh rice brans contained FFA usually between 2.16-2.52%. Total lipid extracts were fractionated into lipid classes by a combination of column and thin layer chromatography. The triglycerides varied from 91.335 to 94.20%, partial glycerides from 1.6% to 4.2% and free sterol from 0.2% to 0.5%. Glycolipid and phospholipid amounted to 0.6%-1.2% and 0.2%-0.5% respectively. The fatty acid compositon of the oils showed that rice bran oil contained 15.00-16.90% of palmitic acid, 2.42-4.20% of steric acid, 45.30-46.62% of oleic acid and 30.28-33.55% of linoleic acid as major fatty acid.  

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Bangladesh Journal of Biochemistry. Vol. 4, No. 1&2, 55-70, 1998
BIOCHEMICAL CHARACTERISTICS OF SOME EDIBLE AND NON-EDIBLE FATS AND OILS OF BANGLADESH.

Nazibur RAHMAN and Harun K. M. YUSUF*
Department of Biochemsitry, University of Dhaka. 

(Received November 30, 1998) 

Abstract
A comparative study was made on some characteristics of edible and non-edible fats and oils available in Bangladesh. Comparison was made between the purified and non-purified forms of the same edible fats and  oils. The parameters based on which the nutritional value of all the fats and oils were determined were iodine value, saponification value, acid value and fatty acid composition. In the case of edible oils, soybean (Postman) had the highest iodine value (149.49) whereas coconut (aromatic) had the lowest iodine value (11.32). Coconut (aromatic) had the highest saponification value (268) whereas ghee (home-made) had the lowest saponification value (211). Butter had the highest acid value (12) whereas palm (Dhaka) had the lowest acid value (0.17). Chromatographic analysis of fatty acids showed that soybean (USA) contained the % highest proportion of  ω-6 fatty acids (54) whereas ghee of the open market contained the lowest (0.89). It was remarkable that coconut (aromatic), ghee (home-made), butter, dalda, mustard of the open market and mustard of ghani did not contain the ω-6 fatty acids. Palm from Chapai Nawabganj, palm from Dhaka, coconut (open market), coconut (aromatic), ghee(open market), ghee (home-made) butter and dalda did not contain  any kind of  ω-6 fatty acids. In the case of non-edible oils, percentage of iodine value was highest in tisi (137.35), whereas chamily had the lowest (1.35). A remarkable feature was that tisi contained such an enourmous proportion of 18:3 (ω-3) fatty acid (38.9) that could not be found normally in any kind of edible oil. 

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Bangladesh Journal of Biochemistry. Vol. 4, No. 1&2, 71-80, 1998
NEEDLE TYPE OXYGEN ELECTRODE FOR THE DETECTION OF OXYGEN TENSION IN TISSUES

Sayed AHMED,
Department of Laboratory Medicine, Institute of Child and Mother Health, Matuail, Dhaka-1362, Bangladesh.
M. Anwar HOSSAIN,
Department of Biochemistry, University of Dhaka, Dhaka-1000, Bangladesh.
and Pankaj M. VADAGAMA
Section of Clinical Biochemistry, Department of Medicine, University of Manchester. UK. 

(Received September 2, 1998) 

Abstract
After a bony fracture or injury, blood vessels are usually damaged and the tissues distal to the injury suffer from ischemia. As a consequence, the bony tissue gradually becomes necrosed and ultimately dies. It is very difficult to pinpoint the area of necrosis, which is essential for the further surgical procedure. The needle type oxygen electrod developed in our laboratory has the potential for the detection of dead tissues. The ability of these electrodes to detect oxygen in buffer sample and its effects on exposure to whole blood and serum are presented.

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Bangladesh Journal of Biochemistry. Vol. 4, No. 1&2, 81-92, 1998
A COMPARATIVE STUDY ON SERUM PROTEINS IN GOITER PATIENTS AND NORMAL INDIVIDUALS 

M. Abdulla HARUN, Mohammad Rezaul KARIM*, Nurul ABSAR and M. SHAHAJAHAN
Department of Biochemistry, University of Rajshahi, Rajshahi-6205, Bangladesh. 

(Received April 1, 1998) 

Abstract
Abnormal serum protein fractions for goiter patient were examined by determining serum total protein, serum albumin, globulin and albumin/globulin ration (A/G) by SDS-polyacrylamide gel electrophoresis. γ -globulin was purified and characterized in both cases of goiter and normal human. The amount of serum total protein increased remarkably (29%)in goiter patients. This increase was mainly due to an abnormal rise in the amount of globulin. The amount of serum albumin was found in the normal ranges between 4.3 and 4.6. α1, α2, γ-globulin increased but β -globulin decreased. γ-globulin was significantly (59%) increased in goiter. The apparent molecular weight of IgG as estimated by SDS-PAGE was 1,58,325. The purified IgG was found to be nonspecific in precipitation by serum of goiter patient (antigen) and in hemagglutination of rat red blood cells.

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Copyright 2003 By The Bangladesh Biochemical Society, Bangladesh.