BANGLADESH JOURNAL OF BIOCHEMISTRY

Bangladesh Journal of  Biochemistry
AN INTERNATIONAL JOURNAL IN BIOCHEMICAL SCIENCE

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Bangladesh Journal of Biochemistry, Vol. 2, No. 1, 1-7, June 1996
DETECTION OF SINGLE COPY HOMOLOGOUS GENOMIC DNA BY THE NON-RADIOACTIVE ENHANCED CHEMILUMINESCENCE METHOD 

Zeba I. SERAJ, Saleh MAHMOOD, Minufer FARZANA and Umme AMARA
Department of Biochemistry, University of Dhaka, Dhaka-1000, Bangladesh. 

(Received March 20, 1996) 

Abstract
The development of non-radioactive methods for detection of single copy homologous sequences in plant or animal genome is of prime importance in developing countries where  monitoring systems for any radioactive contamination, subsequent decontamination and waste disposal are poor or non-existent. Here we report differences and/or similarities between various Oryza sativa L. varieties, homology and nonhomology between O. Sativa L. and Porteresia coarctata T. by using a cloned genomic 1.9 kb fragment of  IR 36 rice as a probe. The probe was labeled with fluorescein and hybridizing bands detected  by Amersham’s indirect enhanced chemiluminescene (ECL) method. The ECL method was also used to check presence/absence of O. sativa and/or P. coarctata bands in some putative hybrids between these two genera.


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Bangladesh Journal of Biochemistry, Vol. 2, No. 1, 9-14,  June 1996
PRODUCTION AND ACTIVITY OF b-1, 3-GLUCANASES DURING GROWTH OF SACCHAROMYCES CEREVISIAE 

Sabita Rezwana RAHMAN, Md. Majibur RAHMAN and Naiyyum CHOWDHURY. Department of Microbiology, University of Dhaka, Dhaka-1000, Bangladesh. 

(Received April 23, 1996) 

Abstract
Two exotic strains of Saccaharomyces cerevisiae DSM-2155, DSM-1848 and one local strain (Gp-1) were studied for both intracellular and extracellular b –1, 3-glucanase production. Local strain GP-1 exhibited maximal intracellular glucanase activity of 7.9 U/ml and extracellular activity of 4.0 U/ml, when the initial pH of the medium was 5.0.b–1,3-Glucanase production was growth associated.

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Bangladesh Journal of Biochemistry, Vol. 2, No. 1, 15-27,  June 1996
SALINITY TOLERANCE AND ACCUMULATION OF NEUROTOXIN AND EXCITATORY AMINO ACIDS IN LATHYRUS SATIVUS
 

Rabiul HOQUE, Muhammad HUSSAIN,
Department of Biochemistry, Bangladesh Agricultural university, Mymensingh.
Yu-Haey KUO and Fernand LAMBEIN
Laboratory of Physiological Chemistry, University of Gent, B 9000 Gent, Belgium 

(Received  June 6, 1996) 

Abstract
Lathyrus sativus (Khesari) was grown in the saline coastal zones, sand culture, artificial saline soils, and liquid nutrient solutions with varying degrees of salinity. Rate of germination, different growth parameters, the neurotoxin b-N-oxalyl-L-a, b-diaminopropionic acid (ODAP) and some other excitatory amino acids of L.sativus  were studied to evaluate relative salinity tolerance. The rate of germination of Khesari seed decreased by 10% to 83% in artificial saline soil with the increase of EC from 3.30 to 12.58 mmohs/cm but germinating capability and seed viability were retained for at least 25 days. The length of shoot continued to increase significantly with the rise of salinity in sand culture and the length of shoot root, number of nodes and 1000 seed weight increased likewise more significantly in saline liquid  nutrient solution containing NaCl up to 0.4% (W/V). The neurotoxin (ODAP) content of L. sativus  green shoot and seed decreased with salinity in the field (up to 16.62 mmohs/cm), that of shoot in artificial saline soils (containing up to 0.1 to 0.5% NaCl and that of green shoot, green pot and embryo in saline hydroponic media containing up to 0.4% added NaCl). The effect of higher salinity on L.sativus (more than 0.4% NaCl) grown in hydroponic nutrient solutions appeared to result from water stress rather than salinity. Salinity in the field experiment (EC 13.33-EC 16.62) also reduced ODAP content of shoot and seed and the rate of decrease was in the shoot. Aspartate content of green shoot top was reduced by salinity while glutamate content was hardly affected by low salinity (up to 0.4% NaCl) but enhanced  by higher salinity. L. sativa, like some rare glycophytes, showed halophillic characteristics by all of its growth parameters which indicates its fairly high salinity tolerance.   

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Bangladesh Journal of Biochemistry, Vol. 2, No. 1, 29-39,  June 1996
THE PROTEASE(S) FROM THE LARVAE OF PULSE BEETLE, CALLOSOBRUCHUS CHINENSIS

Yearul KABIR and Dwaipayan SIKDER

Department of Biochemistry, University of Dhaka, Dhaka-1000, Bangladesh.

(Received  October 26, 1995)

Abstract
Proteolytic activity was detected in the larvae of Callosobruchus chinensis insect. The enzyme had optimal activity at pH 6.0 and 40°C and it preferentially cleaved peptide derivatives with an arginine in the P1  position and a hydrophobic amino acid residue in the P2  position. The enzyme was characterized as cysteine protease, the activity of which was inhibited by Hg+ and activated by EDTA. The protease(s) present in the larvae of C. chinensis exhibited a unique substrate specificity hydrolyzing both Z-Phe-Arg-MCA and Pro-phe-Arg-MCA, which are substrate for mammalian cysteine proteinase, cathepsin B and kallikrein, respectively.   

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Bangladesh Journal of Biochemistry, Vol. 2, No. 1, 41-48,  June 1996
EFFECT OF STORAGE ON THE PHYSICOCHEMICAL CHARACTERISTICS OF SOYBEAN OIL

Hossain U. Shekhar,M. Nazmul ALAM, Zakir H. HOWLADER, and M. Nazmul HUDA,
Department of Biochemistry, University of Dhaka, Dhaka-1000, Bangladesh
M. Amir HOSSAIN
Institute of Statistical Research and Training (ISRT), University of Dhaka, Dhaka-1000, Bangladesh.

(Received June 17, 1996)

Abstract
To monitor the long term storage effect (under different conditions) the most commonly used cooking oil in Bangladesh (soybean) was studied in terms of iodine value, saponification number, acid value, peroxide value and fatty acid pattern. The duration of the study was 270 days. It was observed that during this time iodine value decreased by 30.9%-33.8%, at room temperature (25-40
ºc) and 18.5%-22.1% at freezing temperature (0-4ºc)from the initially recorded value. Saponification value increased by 56.8%-72.0%  and 25.5%-38.5% under the two conditions respectively. Acid value increased by 310%-377% and 144%-292%. Peroxide value increased by 1192%-1785% and 645%-951%, respectively. It was observed that within 90 days of storage the polyunsaturated fatty acids started decreasing and the saturated fatty acid started increasing at a high rate.  

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Bangladesh Journal of Biochemistry, Vol. 2, No. 1, 49-58,  June 1996
ROLE OF NEUROTRANSMITTERS AND NEUROMODULATORS IN CARDIOVASCULAR REGULATIONS

M. Khalilur RAHMAN
Department of Biochemistry, University of Dhaka, Dhaka-1000, Bangladesh

(Received January 4, 1995)

Abstract
Neurotransmitters and neuropeptides are involved in the regulation of nervous function, emotion, sex, sleep, behaviour, and mood of all higher animals, including man. They occur simultaneously in the brain as neurotransmitters or neuromodulators and in plasma as hormones. The direct regulatory interactions of a given substance in the blood and in the brain are not known clearly, but some work has been done on these relationships. The present review briefly discuss the studies on the interrelationship of the brain biogenic amines with cardiovascular regulation. It has been widely investigated to reveal the link between brain catecholamines and baroreceptor reflex control of blood pressure and also in the development of certain types of experimental hypertension. The role of indolamines is to be investigated further as serotonin may exhibit both inhibitory and excitatory effects in the same cell depending on the nature of stimulation. It is also believed that serotonin of the raphé nuclei is mainly involved in the descending inhibitory input to sympathetic ganglion neurons.
It has been shown by many investigators that GABA has inhibitory influence on cardiovascular function. GABA may also interferes centrally with peptidergic blood pressure controlling system. In serum of transgenic mice, the norepinephrine level was reduced and the epinephrine level was slightly elevated. The brain amygdala is important in the integration of cardiovascular adaptive changes. The sympathetic division of the autonomic nervous system acts as an arousal mechanism for the entire body.
Recent evidence indicates that the effect of substance P on blood pressure and heart rate in the result of sympathetic neuron system stimulation. It is possible that vasopressin may operate to interfere centrally with the baroreceptor reflex, most probably by augmenting its responsiveness. There is a stimulated brain rennin-angiotensin system in hypertensive subjects.
                                                                                                   

 

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