BANGLADESH JOURNAL OF BIOCHEMISTRY

Bangladesh Journal of  Biochemistry
AN INTERNATIONAL JOURNAL IN BIOCHEMICAL SCIENCE

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Bangladesh Journal  of  Biochemistry. Vol. 1, No. 2, 55-61, December 1995

EFFECT OF SUBLETHAL DOSE OF COPPER ON QUANTATIVE CHANGES IN TOTAL SERUM PROTEIN OF GOLDFISH, CARASSIUS AURATUS (L.)

M. A. HOSSAIN AND M. SHARIF
Faculty  of Fisheries and Marine Sciences, University Pertanian Malaysia 43400 UPM, Serdang, Selangor, Malaysia.

(Received April 5, 1995)

Abstract
Goldfish Carassius aurutas (average 121.25) were continuously exposed to 10, 30, and 50 _g Cu/I under aquarium conditions. Effect of copper (Cu) on quantitative changes in total serum protein (TSP) of test fish was measured after 2,4 and 6 weeks to compare with the control (Cu unexposed). The TSP of test fish exposed to various sublethal treatments of Cu for different durations were significantly (P<1.01) different from each other and the control. The lowest TSP obtained in control group ranged from 2.08±0.07 to 2.22±0.043 g/dl. The highest amount of TSP (3.11±0.031 g/dl) was obtained from the test fish exposed to 30µg Cu/I after 6 weeks. The Cu exposure-level dependent increase of TSP was observed in all the three treatments which started decreasing at 50µg Cu/I after 6 weeks. The TSP showed significant (P<0.05) positive correlation  to   both  sublethal Cu exposure levels and  exposure time.

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Bangladesh Journal  of  Biochemistry. Vol. 1, No. 2, 63-68, December 1995
EFFECT OF  NICKEL ON SOME HAEMATOLOGICAL AND HUMORAL IMMUNE PARAMETERS IN MICE

BHATIA, N. SOHAL AND M. MAKKAR
Department of Biotechnology, Punjabi University, Patiala, Punjab, India-147002

(Received February 11, 1995)

Abstract
The continuous release of heavy metals at  low levels in the environment is a matter of serious concern because at these levels the individual is exposed to the pollutant at subchronic levels and subtoxic luvels. Hence in the present study we studied the  effect of subchronic, sublethal levels of Ni, a heavy metal, on some haematological and  humoral immune parameters in mice. Our results reveal that Ni exposure at sublethal and subcheonic levels could bring about dose dependent alterations in sole heamatological parameters and affected the development of anti-SRBC and anti-BSA antibody and  also caused a  decline in B lymphocytes. Hence Ni exposee more prone  to infection.

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Bangladesh Journal  of  Biochemistry. Vol. 1, No. 2, 69-72, December 1995
EFFECT OF  SUBCHRONIC SUBLETHAL DOSES OF NICKEL ON SOME  CELL MEDIATED  IMMUNE PARAMETERS

A. BHATIA, N. SOHAL AND M. MAKKAR
Department of Biotechnology, Punjabi University, Patiala, Punjab, India-147002

(Received February 11, 1995)

Abstract
The effect of subchronic, sublethal doses of nickel chloride has been studied on some cell mediated immune  parameters viz. leukocyte migration inhibition, leukocyte adherence inhibition and NBT reduction. The Ni exposure at subchrnic and  sublethal doses  results in  a   dose dependent depression in T  cell mediated function but activitates  the  phagocytes.

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Bangladesh Journal of Biochemistry. Vol. 1, No. 2, 73-78, December 1995
CROTEPOXIDE AND N-BENZOYL-O-(N´-BENZOYL-L-PHENYLALANYL)-L-PHENYLALANINOL FROM THE STEM BARK OF DESMOS LONGIFLORUS

Md. Enamul HAQUE, and Adil MUTTAQUIN
Department of Biochemistry, University of Dhaka, Dhaka 1000, Bangladesh
Josheph D. CONNOLLY
Department of  Chemistry, University of Glasgow G12 8QQ, Scotland, U. K.

(Received September 30, 1995)

Abstract
Crotepoxide a cytotoxic cyclohexane derivatives and  N-benzoyl-O-(N´-benzoyl-L-phenylalanyl)-l-phenylalaninol, an N-benzoyl ester of phenylalanine were isolated from the ethyl acetate extract of the  stem bark of  Desmos longiflorus (Annonaceae). The structures of  these compounds were  determined by  extensive spectroscopic analyses (UV, IR, H  NMR, 13C NMR and EIMS). This is  the  first report of the  presence of N-benzoyl-O-(N´-benzoyl-L-phenylalanyl)-L-phenylalninol  i9n an Annonaceous plant. Preliminary screenings have shown that this compound also possesses cytotoxic activity.

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Bangladesh Journal  of  Biochemistry. Vol. 1, No. 2, 79-85, December 1995
PURIFICATION AND MOLECULAR WEIGHT DETERMINATION OF PROTEIN-DEMIDASE FROOM GERMINATING SEEDS 

R. K. SHAHA, and  I. A.  VAINTRAUB
Deoartment of Biochemistry, Rajshahi University,   Rajshahi, Bangladesh
L. V.  KATOVA
Laboratory of Protein chemistry, Moldavian University, Kshnev-277014, republic of Moldova

(Received April 29, 1995)

Abstract
Enzymes with deamidating activity from germinating wheat grains was purified about 570-fold first by chromatography on  hydroxylapatite and then  on DEAE-cellulose columns. When this procedure was applied to  the deamidase of kidney beans and squash the degrees of purification were about 210 and 250-fold and the specific activities were about 770 mU/mg and 880mU/mg respectively. Polyacrylamide gel electrophoresis showed the purified deamidase from the above three sourcrs to be homogeneous, with apparent molecular weight of 18,000.

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Bangladesh Journal  of  Biochemistry. Vol. 1, No. 2, 87-91, December 1995
OCCURRENCE AND PROPERTIES OF EXTRACELLULAR a-MANNANASE  OF  SACCHAROMYCES CEREVISIAE STRAIN DSM 2155 

Sabita R. RAHAMAN, M. Majibur RAHAMAN and Naiyyum CHOWDHURY
Department of Microbiology, University of Dhaka, Dhaka 1000, Bangladesh

(Received August  26, 1995)

Abstract
Saccharomyces cerevisiae strain DSM 2155 produced extracellular a-mannanase in MYGP medium in a growth associated manner. The enzyme activity was optimal at 40°C and pH 5.0. the Km of the enzyme was 0.6 mg mannan per ml and the Vmax was 3.8 U/mg protein.
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Bangladesh Journal of Biochemistry. Vol. 1, No. 2, 93-101, December 1995
HIGH PERFORMANCE LIQUID CHROMATOGRAPHIC ANALYSIS OF EXCITATORY AMINO ACIDS IN LATHYRUS SATIVUS PLANTS AND SEEDS 

Rabiul HAQUE, Farnand LAMBEIN, and Muhammed HUSSAIN
Department of Biochemistry, Bangladesh Agricultural University, Mymensingh, Bangladesh
Fernand LAMBEIN, Yu-Haey KUO, J. Kabir KHAN
Laboratory of physiological  Chemistry, University of Ghent, B-9000 Ghent, Belgium

(Received December 22, 1995)

Abstract
The excitatory amino acids aspartate, glutamate and  ß-N-oxalyl-L-a, ß-diaminopropionic acid (ODAP) were determined in  green shoot top and seed of Lathyrus sativus by high performance liquid chromatographic (HPLC) technique. The  amino acids  were derivatized with phenylisothiocyanate (PITC) and the   PITC-amino acid derivatives were determined by C18 reversed  phasecolumn and detected by UV detector. Integration of the signals was done by  a   Hewlett Packard Integration system. The  efficiency of the HPLC method was compared with that  of  an  amino acid  analyzer. The HPLC method was found tohave a recovery of 92.2%.

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