BANGLADESH JOURNAL OF BIOCHEMISTRY

Bangladesh Journal of  Biochemistry

AN INTERNATIONAL JOURNAL IN BIOCHEMICAL SCIENCE

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Bangladesh Journal  of  Biochemistry. Vol. 1, No. 1, 1-8, June 1995

Tumour vaccine activity of 64KDa Stress protein derived from myocobacterium bovis bcg

Jin-ichi SASAKI,
Department of Bacteriology, Hirosaki University School of Medicine, Hirosaki 036, Japan
Kris HUYGEN, Michel DEJEHANSART,
Institute Pasteur du Brabant, Brussels, Belgium.
Chowdhury R.AHSAN, Tetsutaroh ONO,
Department of Bacteriology, Hirosaki University School of Medicine, Hirosaki 036, Japan.
and Jacqueline De BRUYN
Institute Pasteur du Brabant, Brussels, Belgium.  

(Received October 17, 1994)  

Abstract
Experiment was carried out to examine the tumour vaccine activity of Mycobacterium bovis BCG HSP64 in Meth A tumour model of BALB/c mouse. This antigen was broadly distributed among animal and human types of tumor cells likely to be a tumor-associated antigen. Mice were immunized with BCG HSP64 (10 mg per mouse) three times at one week interval in advance. Meth A tumor cells were intradermally transplanted. The vaccinated mice inhibited tumor growth at 30%, indicating the induction of tumor protective immunity. The increasing production of antibody was observed in serum at high level of titer (104-105 dilution times) in vaccinated mice. Further, the levels of cytokines  such as IL-2,IL-6,IFN-g,or TNF-a were also elevated in spleen cell culture supernatants  or peritoneally exudated cells originated from BCG HSP64 vaccinated mice in response to BCG HSP64 stimulant. These results suggests that the BCG HSP64 possibly possesses tumour vaccine activity by enhancing production of antibody and cellular immunity.

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Bangladesh Journal  of  Biochemistry. Vol. 1, No. 1, 9-15, June 1995
CHOLESTEROL LOWERING EFFECT OF HILSHA FISH OIL IN FEMALE RATS

Sushil SINHA and Rafiqur RAHMAN
Department of Biochemistry, University of Dhaka, Dhaka-1000, Bangladesh.

(Received November 13, 1994)  

Abstract
The body oil of hilsa (Tenualosa ilisha), a fatty riverine tasty fish of Bangladesh, when fed to hypercholesterolemic female rats, dramatically reduced their serum cholesterol level. The oil feeding had significantly reduced both serum VLDL and LDL-bound cholesterol while elevating the HDL-bound cholesterol. Consistent with this, the HDL-LDL ratio which was depressed by cholesterol feeding, was restored by oil feeding.
Assay of the hepatic HMG-CoA reductase, the rate limiting enzyme of cholesterol biosynthesis, revealed that oil feeding had significantly depressed its activity manifested by a relatively higher HMG-CoA/MVA ratios found in the livers of the oil-fed animal. These results indicate that one mechanism by which hilsa oil reduces blood cholesterol is by inhibiting endogenous synthesis of cholesterol
.

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Bangladesh Journal  of  Biochemistry. Vol. 1, No. 1, 17-22, June 1995
INTRINSIC RESISTANCE OF LEUKEMIA CELLS TO AN ANTICANCER DRUG

M. Perwaiz IQBAL  
Department of Biochemistry, The Aga Khan University, Stadium Road, Karachi-74800, Pakistan.  

(Received December 13, 1994)

Abstract
A number of mechanism have been shown to be operative in mammalian cells for the development of resistance to the anticancer drug, methotrexate. Recently, there have been reports of the intrinsic resistance of certain leukemia cells to methotrexate. Using L1210 leukemia cells, we have shown that this intrinsic resistance to methotrexate is due to a form of dihydrofolate reductase having low affinity for this drug.

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Bangladesh Journal  of  Biochemistry. Vol. 1, No. 1, 23-32, June 1995
TOTAL WHITE BLOOD CELL COUNT AND PHAGOCYTIC ACTIVITY OF THE NEUTROPHILS IN MONKEYS WITH EXPERIMENTAL SHIGELLOSIS

Anwarul FERDOUS and Laila N. ISLAM  
Department of Biochemistry, University of Dhaka, Dhaka-1000, Bangladesh.
 

(Received January 10, 1995)

Abstract
The effect of experimental shigellosis upon the total white blood cell count and the phagocytic activity of neutrophils was studied in monkeys on different days after oral challenge with Shigellae. Fifteen monkeys were first challenged with S. dysenteriae type 1 which caused shigellosis in nine monkeys. After recovery, the monkeys were challenged again with S. flexneri 2a. There were marked changes in the sensitivity in the animals and also in the phagocytic activity of their neutrophils during shigellosis compared to unchallenged controls. However, no difference in the effects was observed in the monkeys during shigellosis caused by either S. dysenteriae 1 or S. flexneri 2a. The WBC count decreased slightly within the first week of challenge, increased during the second and third weeks but ultimately returned  to the normal level while the animals recovered. The proportion of neutrophils phagocytosing opsonized yeast varied between 68 and 96% but that for unopsonized yeast were 2-8%, in normal  healthy monkeys. The corresponding values were 96-99% and 12-50%, respectively on day 6 of challenge. Also, the number of opsonized yeast phagocytosed by one hundred neutrophils was 446-796 in normal healthy monkeys but 900-980 in challenged monkeys on day 6. Neutrophils from monkeys ill with shigellosis were found to be highly activated as they phagocytosed more yeast particles.

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Bangladesh Journal  of  Biochemistry. Vol. 1, No. 1, 33-40, June 1995
DEGRADATION OF SOLUBLE SERUM PROTEINS IN HEVEA BRASILIENSIS LATEX BY BOTTOM FRACTION ACID PROTEASE

Kamruzzaman AMPON, Abdullah SIPAT and Mohd. Arif SYED

Department of Biochemistry and Microbiology, Faculty of Science and Environmental Studies, Universiti Pertanian Malaysia, 43400 UPM Serdang, Selangor Darul Ehsan, Malaysia.  

(Received October 17, 1994

Abstract
The degradation of soluble serum protein mixtures by proteases that are present in the bottom fraction lutoids of Hevea brasiliensis latex was investigated to assess their possible involvement in in vitro protein turnover. The degradation showed a pH optimum of about 3.5. The strong inhibition by pepstatin suggested that acid protease may be the major proteolytic enzyme involved. The serum proteins were degraded less rapidly after denaturation compared to the native state. There was a good correlation of digestibility with the molecular sizes of the protein substrates used.

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Bangladesh Journal  of  Biochemistry. Vol. 1, No. 1, 41-46, June 1995
ISOLATION AND PURIFICATION OF LECTIN FROM GARDEN PEA ( PISUM SATIVUM L.)

M. Shamsul HOQUE, Balaram ROY, M. M. NURUDDIN and M. Abdul HAMID  
Department of Agricultural Chemistry, Hajee Mohammad Danesh Krishi College, Dinajpur, Bangladesh.  

(Received October 10. 1994)  

Abstract
A method is described for the isolation and purification of a lectin from garden pea (Pisum sativum) by affinity chromatography on Sephadex G-150 column. The purified lectin has an apparent molecular weight of 49,000. It showed haemagglutination (2% erythrocytes) at a concentration of less than 4 mg/ml and caused 100% mortality of the larvae of Callosobruchus maculatus at a concentration of 2.5%.

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Bangladesh Journal of Biochemistry. Vol. 1, No. 1, 47-52, June 1995
FORMATION OF HISTAMINE IN CHLORPROMAZINE TREATED GUINEA-PIGS

B. H. RIDZWAN
Department of Biomedical, Faculty of Allied Health Sciences, Universiti Kebangsaan Malaysia, 50300 Kuala Lumpur, Malaysia.  

(Received November 2, 1994) 

Abstract
Oral administration of
14C-histidine induced the presence of 14C-histamine in certain tissues of both control and chlorpromazine (CPZ)-treated (30 mg/kg body wt., i. p.) guinea-pigs. The mean 14C-histamine contents in the tissues of the CPZ-treated animals were found slightly lower than in the control animals. However, the amounts formed were very small in comparison to the endogenous histamine contents of the tissues.14C-histidine induced the presence of 14C-histamine in certain tissues of both control and chlorpromazine (CPZ)-treated (30 mg/kg body wt., i. p.) guinea-pigs. The mean 14C-histamine contents in the tissues of the CPZ-treated animals were found slightly lower than in the control animals. However, the amounts formed were very small in comparison to the endogenous histamine contents of the tissues.

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